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Is the process of covalently link two DNA ends. The process is usually done by the enzyme class of ligases.
3
votes
What are common causes of unexpected ligation products?
Could be insert polymerization. If you have your stretch of DNA like this:
(5')-AATTagctagcatcgtgatcgacg-(3')
||||||||||||||||||||
(3')-tcgatcgtagcactagcagcGGCC-(5')
And you take t …