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The genetic alteration of a cell resulting from the direct uptake, incorporation, and expression of exogenous genetic material from its surroundings.

1 vote

Problem with bacterial transformation with electroporation

I did try another transformation: again with our electrocompetent cells Cells form other lab chemically competent cells I made The good thing is that on chemically competent cells there is no weir … colonies, however efficiency of transformation was very low, but still, I think it is ok - will confirm it in next day if I really got the right insert. …
MartinK's user avatar
  • 338
3 votes
1 answer
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Problem with bacterial transformation with electroporation

I have a problem with a bacterial transformation of a yeast gene that I can not solve. I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. …
MartinK's user avatar
  • 338