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The genetic alteration of a cell resulting from the direct uptake, incorporation, and expression of exogenous genetic material from its surroundings.
1
vote
Problem with bacterial transformation with electroporation
I did try another transformation:
again with our electrocompetent cells
Cells form other lab
chemically competent cells I made
The good thing is that on chemically competent cells there is no weir … colonies, however efficiency of transformation was very low, but still, I think it is ok - will confirm it in next day if I really got the right insert. …
3
votes
1
answer
2k
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Problem with bacterial transformation with electroporation
I have a problem with a bacterial transformation of a yeast gene that I can not solve.
I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. …