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Spelling, formatting, changed title to just state the problem, not as a protocol request
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edit approved Dec 22, 2011 at 16:53
Mad Scientist
edit approved Dec 22, 2011 at 16:53
Mad Scientist
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What is a good protocol for measuting How can I measure bacterial Alkaline Phosphatasealkaline phosphatase activity?

I want to measure Alkaline Phosphatasealkaline phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the Phosphatasephosphatase (which I have no need of). Does

Does anyone know a good protocol which involves using only the supernatant, without purification of the enzyme? What is the preferablepreferred buffer for the reactireaction?

What is a good protocol for measuting bacterial Alkaline Phosphatase?

I want to measure Alkaline Phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the Phosphatase (which I have no need of). Does anyone know a good protocol which involves using only the supernatant? What is the preferable buffer for the reacti

How can I measure bacterial alkaline phosphatase activity?

I want to measure alkaline phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the phosphatase (which I have no need of).

Does anyone know a good protocol which involves using only the supernatant, without purification of the enzyme? What is the preferred buffer for the reaction?

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Innab
Innab
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What is a good protocol for measuting bacterial Alkaline Phosphatase?

I want to measure Alkaline Phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the Phosphatase (which I have no need of). Does anyone know a good protocol which involves using only the supernatant? What is the preferable buffer for the reacti