Timeline for How do I make conclusions from the autoradiograph of a Southern blot?
Current License: CC BY-SA 3.0
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| Apr 19, 2015 at 1:21 | comment | added | mdperry | A Southern blot needs to be probed with labeled DNA. It is not clear from your description what the origin of such a probe would be. Nor are Southern blots typically used to resolve and detect such short pieces of DNA--in fact you would need an extremely high percentage of agarose in a gel to achieve this resolution. That suggests it is actually a polyacrylamide gel, and therefore unlikely to be a Southern blot. The fact the the primers match sequences in the Human genome is irrelevant. The question is about using VTNR as dominant genetic markers to "fingerprint" genomic DNA samples. | |
| Apr 18, 2015 at 17:04 | vote | accept | user170231 | ||
| Apr 18, 2015 at 12:06 | answer | added | Nandor Poka | timeline score: 2 | |
| Apr 18, 2015 at 8:18 | comment | added | user170231 | Okay that makes much more sense, thanks for clarifying. | |
| Apr 18, 2015 at 8:08 | comment | added | canadianer | For your first question, the sentence could be rewritten as: "are on either side of highly polymorphic sites in human DNA that result from variable numbers of tandem nucleotide repeats." | |
| Apr 18, 2015 at 8:00 | history | asked | user170231 | CC BY-SA 3.0 |