Anaerobic bacteria utilise glycolysis:

Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate

followed by fermentation:

Pyruvate + NADH => Lactate + NAD+

The resultant NAD+ formed can then be used again to break down more glucose molecules in glycolysis to produce ATP.

However, besides NAD+, glycolysis also requires an input of 2 ATP at the beginning (for the phosphorylation of glucose to glucose 6P, and fructose 6P to fructose 1,6 BP)

Since only 2 ATP is produced from glycolysis initially, would these 2 ATP be reused in another round of glycolysis?

If so, how does anaerobic bacteria produce a net amount of ATP for cell activities and growth?

Anaerobic bacteria utilise glycolysis:

Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate

followed by fermentation:

Pyruvate + NADH => Lactate + NAD+

The resultant NAD+ formed can then be used again to break down more glucose molecules in glycolysis to produce ATP.

However, besides NAD+, glycolysis also requires an input of 2 ATP at the beginning (for the phosphorylation of glucose to glucose 6P, and fructose 6P to fructose 1,6 BP)

Since only 2 ATP is produced from glycolysis initially, would these 2 ATP be reused in another round of glycolysis?

If so, how do anaerobic bacteria produce a net amount of ATP for cell activities and growth?