Timeline for Understanding the strategy of Sanger DNA sequencing
Current License: CC BY-SA 4.0
9 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| Sep 23, 2019 at 12:09 | history | edited | David | CC BY-SA 4.0 |
Corrected typo in title.
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| Sep 23, 2019 at 12:06 | answer | added | David | timeline score: 1 | |
| Sep 14, 2019 at 9:00 | history | tweeted | twitter.com/StackBiology/status/1172797260524130304 | ||
| Sep 13, 2019 at 0:29 | vote | accept | user9807 | ||
| Sep 12, 2019 at 16:04 | comment | added | swbarnes2 | > why not just do regular PCR with them, create a huge number of full > length copies of the original sequence, and then simply see the > locations where the nucleotide fluoresces to determine all the > locations of that base. How? What instrument do you propose to use to see the sequence of fluorescent tags? | |
| Sep 12, 2019 at 13:18 | answer | added | Chris♦ | timeline score: 5 | |
| Sep 12, 2019 at 9:46 | comment | added | David | It is unclear whether you are asking a) how the Sanger method works, or b) why it is used (or why it was invented) when Next Gen methods are better. However my guess is that you are student who has encountered DNA sequencing methods without any historical or practical context, and has got bogged down in the preparation of the samples rather than the methods for actually determining the sequences. Read Wikipedia on Sanger and more modern Sequencing methods. (The Sanger method predates PCR.) | |
| Sep 12, 2019 at 2:20 | review | First posts | |||
| Sep 12, 2019 at 17:47 | |||||
| Sep 12, 2019 at 2:15 | history | asked | user9807 | CC BY-SA 4.0 |