Skip to main content

All Questions

Filter by
Sorted by
Tagged with
3 votes
1 answer
123 views

T-vector creation

I'm working on TA cloning for the first time and I need to turn some existing plasmid backbones into T-vectors. I'm using a Klenow fragment (3' -> 5' exo-) to dA-tail my inserts. Is there any ...
Kat's user avatar
  • 33
6 votes
1 answer
975 views

How can I convert plate reader measurements of Pichia pastoris OD to cells per ml?

I am performing experiments with the yeast Pichia pastoris where it will be beneficial to calculate product yield per cell. I plan to use a plate reader to measure OD600 of my cultures and then ...
Noah Sprent's user avatar