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Polymerase Chain Reaction is a method of replicating DNA exponentially from even a single molecule.
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too low extention time for Taq polymerase PCR
I am currently working on my masters degree and have recieved the following question:
"you want to amplify by PCR a gene 1kb long. … You make a mistake in programming the PCR machine and set the extension time to 30 seconds instead of 1 minute. what will happen? what do you expect to see on a gel?" …