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The study of the molecular processes underlying life.
4
votes
What are good resources for self-learning modern molecular biology concepts?
Drop by the diybio.org mailing list, which is home to a bunch of autodidactic free thinkers like yourself. They always can suggest fun projects and learning resources.
Also check out Nature's Scitab …
5
votes
Accepted
Has anyone tried Gibson Assembly Optimizations?
I haven't tried Gibson Assembly myself, but I do know the Cambridge iGEM Team in 2010 and 2011 developed a web-based tool - Gibthon - for automatically generating primers for Gibson Assembly.
You cou …
2
votes
Accepted
How to secrete a recombinant protein from E. coli?
According to the 2012 iGEM Kyoto team:
The Twin Arginate Translocation pathway(TAT) is [an endogenous] secretion system in E.coli. This system can carry proteins that have torA signal amino acid s …