Skip to main content
Search type Search syntax
Tags [tag]
Exact "words here"
Author user:1234
user:me (yours)
Score score:3 (3+)
score:0 (none)
Answers answers:3 (3+)
answers:0 (none)
isaccepted:yes
hasaccepted:no
inquestion:1234
Views views:250
Code code:"if (foo != bar)"
Sections title:apples
body:"apples oranges"
URL url:"*.example.com"
Saves in:saves
Status closed:yes
duplicate:no
migrated:no
wiki:no
Types is:question
is:answer
Exclude -[tag]
-apples
For more details on advanced search visit our help page
Results tagged with
Search options not deleted user 51

The study of the molecular processes underlying life.

4 votes

What are good resources for self-learning modern molecular biology concepts?

Drop by the diybio.org mailing list, which is home to a bunch of autodidactic free thinkers like yourself. They always can suggest fun projects and learning resources. Also check out Nature's Scitab …
Mac Cowell's user avatar
5 votes
Accepted

Has anyone tried Gibson Assembly Optimizations?

I haven't tried Gibson Assembly myself, but I do know the Cambridge iGEM Team in 2010 and 2011 developed a web-based tool - Gibthon - for automatically generating primers for Gibson Assembly. You cou …
Mac Cowell's user avatar
2 votes
Accepted

How to secrete a recombinant protein from E. coli?

According to the 2012 iGEM Kyoto team: The Twin Arginate Translocation pathway(TAT) is [an endogenous] secretion system in E.coli. This system can carry proteins that have torA signal amino acid s …
Mac Cowell's user avatar