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Results tagged with gel-electrophoresis
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user 96
A method for analyzing or purifying samples. Molecules are separated by their relative weight, charge, or stereochemistry, which affect their speed of movement through a gel.
2
votes
1
answer
1k
views
Where does Taq polymerase migrate during electrophoresis?
Assuming a 1% Agarose gel with TAE.
Follow up questions:
Is there a way to stain for it?
Could the polymerase be captured and reused?
4
votes
1
answer
107
views
Is there a cheap and easy way to check for potentially unsafe UV exposure?
My lab has an older large Fotodyne tabletop UV transilluminator for visualizing gels. It has a UV blocking cover that hopefully reduces UV that passes through, but I've always been suspcious as to ho …
7
votes
Alternatives to ethidium bromide for staining small nucleic acids?
I would think GelRed or GelGreen would be an option too. They claim to be more sensitive than EtBr and certainly less toxic (even moreso than SYBR Green). I haven't personally used them against such …