Skip to main content

Questions tagged [experimental-design]

Questions on how to plan an experiment to gain the best results. Also suitable for questions on why other researchers followed a certain procedure.

Filter by
Sorted by
Tagged with
3 votes
0 answers
60 views

How to measure the ability to discriminate between two colors experimentally

CONTEXT Species differ greatly in their vision ability in general, and in their ability to discriminate between colors in particular. Humans' color vision is usually (some rare human individuals have ...
J..y B..y's user avatar
  • 224
-1 votes
1 answer
75 views

Why are there error bars for zero concentration in some cell viability graphs, if they are assumed to be 100%?

Take these for example: As far as I know, the formula for cell viability is % Viability = (Treatment OD - Blank OD) / (Control OD - Blank OD) where each OD is the average of three wells (technical ...
h088bmIuXaskpzJEe3ld's user avatar
1 vote
1 answer
56 views

Behavioural experiment on Nasonia Wasps

I want to see how two male Nasonia vitripennis interact with each other. I have been using enamel paint on their heads to distinguish them, but I am wondering if any other methods of marking them ...
Aavani Babu's user avatar
2 votes
0 answers
109 views

Does binge watching cause sleep problems?

According to a study from 2017, binge watching is correlated with various sleep problems: The people who reported binge-watching were 98 percent more likely to report poor sleep quality compared with ...
Riemann's user avatar
  • 235
3 votes
0 answers
67 views

Why am I getting a large horizontal band spreading across my native gel?

On every gel of this type that I've ran so far, I'm getting this large band interfering with my gel analysis no matter how much troubleshooting I've done. This is a Novex Wedgewell 6% Tris-Glycine Gel ...
novara's user avatar
  • 31
0 votes
1 answer
614 views

Which plants are best for experiments about plant growth? [closed]

I was assigned writing a term paper about optimizing plant growth using control systems. This assignment is part of a class on sustainable energy usage, which means it's primarily on physics and ...
deck4rd's user avatar
  • 17
2 votes
1 answer
64 views

What is the meaning of NF-kB (transcription factor) "activity"?

This article discuss about the NF-kB activity and its mathematical modeling Understanding NF-κB signaling via mathematical modeling. The NF-kB is a transcription factor mainly involved in the ...
Manuela's user avatar
  • 443
1 vote
1 answer
103 views

Is it viable to make liquid potassium ferilizer through dissolving very small amounts of potassium chloride in water

I wasn't able to find fertiliser online that was simply potassium chloride and I didnt want to source my potassium chloride solution from potassium sulphate or other general NPK fertilisers for my ...
Malhar Kookada's user avatar
2 votes
1 answer
2k views

Cell culture experiments, technical or biological replicates?

Experimental design: Cells are grown, counted, and seeded in 96-well plates. Cells recover 24 hours. Cells are treated with different doses of a drug, 3x per treatment. Cell survival read via MTT. ...
Bryan's user avatar
  • 181
1 vote
0 answers
15 views

Is there any reagent that can help me obtain the protein in a 14 μm tissue without damaging the morphology of the tissue

I need to extract proteins, but I want to protect the morphology of the tissue. Can I use lysis buffer directly?
Ming's user avatar
  • 31
1 vote
1 answer
55 views

What is a "marker-matched" plasmid?

I see this term used a lot in papers and have trouble understanding it. From what I have gleaned it seems they are used during control experiments, perhaps with the goal of making the control plasmids ...
k_1223's user avatar
  • 35
0 votes
0 answers
33 views

PCR: From transgene/microgram to cell concentration

I am doing research on CAR T-cell kinetics. The measurement of CAR T-cell concentrations across time is normally carried out with qPCR (see here, Fig. 1). These concentrations are generally reported ...
amr95's user avatar
  • 1
1 vote
0 answers
31 views

Is there a good protocol for bilateral decortication in rodents?

I was thinking about using bilateral decortication in rodents (preferentially mice) to study the roles of certain brainstem areas in cognitive tasks in the absence of cortex. However almost all ...
Matheus C.'s user avatar
1 vote
1 answer
114 views

What are some experimental techniques to identify binding partners?

What experimental techniques can be used to identify binding partners for a given protein? I know that if you have some candidates that may bind to a given protein, then you can use techniques such as ...
Cactus's user avatar
  • 19
0 votes
1 answer
29 views

Can disk diffuson be used, without diluting a broth culture to match a 0.5 McFarland turbidity standard?

In this video, there doesn't seem to be a process of diluting the broth. I'm not looking for extremely accurate test results, all I need is to see if a particular colony is susceptible to the ...
John Greene's user avatar
25 votes
1 answer
3k views

Why are drug dosages so high in some mice studies?

On reading through some research on the effects of certain drugs I often come across staggeringly high dosages such as in this paper: Römer, B., Pfeiffer, N., Lewicka, S., Ben-Abdallah, N., Vogt, M. ...
kcpm's user avatar
  • 353
2 votes
0 answers
25 views

Vegetation density estimation from distance method: random sampling or along the grid?

I am planning to sample the tree regeneration (new ones and advance regeneration, > 2m). I am planning to use small circular points to estimate the counts per area (= density of young trees). I ...
maycca's user avatar
  • 257
0 votes
0 answers
44 views

I am conducting an experiment with oil eating bacteria. However, I am struggling to find a method to check how much oil they cleaned?

I am experimenting to determine the optimum conditions for microbes to break down motor oil. The conditions I am going to test are different pH levels to resemble freshwater pH and saltwater pH. I ...
Gaussian 123's user avatar
0 votes
1 answer
40 views

Would it be possible to genetically modify a plant to produce usable antibodies? And if eaten would they be used?

I am thinking of a project where i would Gene edit a plants cells to produce anti bodies that humans could use. Does anyone know A. what genes to modify? B. Would the antibodies be usable after ...
Astrophysicsteen's user avatar
1 vote
1 answer
55 views

What is a (preferably) flying carnivorous insect that hunts adult fruit flies at a rate that doesn't threaten extinction?

I am forming an experiment, where I will be forcing evolution on Fruit Flies (Thanks rogue ant) The experiment goes a bit like this; I will be forming an ecosystem where every abiotic factor is ...
Astrophysicsteen's user avatar
1 vote
1 answer
59 views

What insect/invertebrate species evolves fastest?

I am starting an experiment in which I will be forcing evolution in a moderately complex species of insect or invertebrate. I am prepared for the possible longevity of this experiment, but i have no ...
Astrophysicsteen's user avatar
1 vote
1 answer
538 views

how to extract garlic fructan?

can anyone tell me a not complicated way to extract fructans from garlic (i have access to highschool lab). I need this to be able to make an experiment on garlic extract and just fructan from garlic ...
Agne's user avatar
  • 19
0 votes
0 answers
19 views

Is there a way to use presence-absence data to inform a sampling design for a more specific survey?

I am currently in the process of designing a spatial survey to assess the abundance/density of woody debris across an ecosystem with multiple defined habitats (i.e. I am interested in not only the ...
mchen's user avatar
  • 1
1 vote
2 answers
92 views

Ensuring all individuals are genetically identical before experiments

I am a beginner in experiment design so please correct me if I made any mistakes. If we want to study the behavioral changes after a particular mutation of a gene, it seems to be a must for all ...
Questions's user avatar
  • 371
0 votes
1 answer
400 views

How do peroxisomes work?

I have this question from my book: A child is diagnosed with an enzyme deficiency that prevents the production of hydrogen peroxide. What would the likely outcome be of such deficiency? H2O2 is ...
Ibby's user avatar
  • 45
0 votes
0 answers
95 views

Relationship between BMI and age?

I'm trying to study how age as a confounder may affect the relationship between commute mode and BMI (and design a research proposal). To explain age as a potential confounder, I compared age to ...
Janey's user avatar
  • 1
0 votes
1 answer
43 views

Antimicrobial susceptibility testing

To learn the Kirby-Bauer method, I've been looking at youtube videos where a bacteria's susceptibility is tested. In this video, the researcher places the discs (containing antibiotics) right after ...
ninetysix's user avatar
0 votes
1 answer
90 views

Can I imbibe seeds before sterilization?

For an experiment I want to germinate lettuce seeds. For logistical reasons it would be convenient to imbibe the seeds by placing them in the cold for 2 days, afterwards I would sterilize the seeds ...
lucas van der zee's user avatar
2 votes
0 answers
16 views

Most optically transparent muscle phantom?

I am trying to use Schlieren imaging techniques to visualize how a sound wave propagates through muscle tissue. In the past, when needing to experiment with muscle we would use an agar based muscle ...
Landon's user avatar
  • 133
0 votes
1 answer
79 views

What material is permeable for plant roots, but "impermeable" for fungi & bacteria?

I'm designing an experiment where the roots of the plant will grow in a sterile environment while the shoot will grow in a non-sterile grow room. For this reason the environments of the shoot and the ...
lucas van der zee's user avatar
2 votes
1 answer
108 views

Question in gel chromatography experiment

Here I am trying to do gel chromatography to separate vitamin B12 and Bovine Serum Albumin (BSA) in this experiment, I am using sephacryl s-100 HR gel column my question is can I use Phosphate ...
HAYA's user avatar
  • 21
0 votes
0 answers
29 views

Problem with epoxy silane (Random results in glass silanization). What are crucial points to look at?

I`m trying to immobilize an antibody onto the glass surface by means of GPTOMS (glycidoxypropyl trimetoxy silane). Using the same protocol (silanization in toluene or acidified ethanol (see below)) I ...
Hedgehog's user avatar
2 votes
2 answers
83 views

How would one track a mineral nutrient in a plant in order to determine if the nutrient has been remobilized?

Before abscission, senescence of a plant’s structural components that contain mineral nutrients (E.g. Magnesium, Potassium...) are re-mobilized from the senescent tissue and used in other plant tissue ...
Gunjit Glen's user avatar
3 votes
2 answers
69 views

Finding total concentration of enzymes

Sorry if my question is very basic for biology majors because I am not. I am trying to build a mathematical model of a particular pathway using systems of differential equations and in order to reduce ...
TheLast Cipher's user avatar
0 votes
1 answer
258 views

Is there anyway from which we can measure the viscosity of honey using a refractometer?

Is it possible to use a refractometer to measure the viscosity of honey because as we know, a refractometer is used to measure moisture and therefore water influences the viscosity of honey so is ...
Mo Kamal's user avatar
2 votes
2 answers
144 views

Monitoring fish growth

I am new to experimental design and would like to perform the following experiment but I am not sure if my setup is correct or not so far. My idea is below. Let's say you want to optimise the growth ...
RUNN's user avatar
  • 21
4 votes
1 answer
118 views

Selection marker for a cytoplasmic vector that works at low expression levels?

I need to express a selection marker on a plasmid in human cells (e.g. HEK293). However, I am using a cytoplasmic vector derived from a non-human virus with its own mechanism for transcriptional ...
Mowgli's user avatar
  • 1,929
0 votes
1 answer
45 views

What slope do I need to prove a hypothesis? [closed]

I am conducting an experiment for school in which I am measuring people's heart rates as music plays at different tempos. My hypothesis is that as the tempo of the music increases, so will the subject'...
caleb lee's user avatar
  • 149
0 votes
0 answers
44 views

Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
jack's user avatar
  • 221
3 votes
1 answer
49 views

Is there an adhesive that could used for larval fish?

I am currently working on an experimental design that requires a larval fish (~ 4mm long) to be affixed on a metal wire tip on its dorsal side. Now, I was thinking of a glue or adhesive to do the job, ...
Macx Jigger's user avatar
3 votes
2 answers
652 views

Will amylase inhibitors affect the colorigenic reaction between starch and iodine?

I'm doing an experiment for my IB bio EE involving colorimetry. I'm not experienced at all with colorimetry, so I'm having some trouble planning it. The experiment is on enzyme kinetics, and I'm ...
coloursofmadness's user avatar
0 votes
1 answer
86 views

Why didn't these scientists restore electrical activity in this pig's brain?

This experiment was published in Nature Magazine: Pig brains kept alive outside body for hours after death. The researchers used a system called BrainEx to revive certain metabolic and physiological ...
Patrick's user avatar
  • 25
0 votes
0 answers
30 views

Why don't materials and methods describe culture media in volume?

After reading several materials and methods sections of cell culture experiments, I have not seen the recipe for culture media ever described in terms of volume. Human adipose tissue was obtained ...
libby's user avatar
  • 121
0 votes
1 answer
43 views

Computational approaches for making hypotheses about the effects of genetic engineering? Experiment planning methods?

Let's assume that I am searching for gene editing candidates for curing human adiposis. Are there computational frameworks that can allow me to select the best candidate-genes for editing via some ...
TomR's user avatar
  • 103
0 votes
1 answer
265 views

How can I estimate the total number of yeast cell in a medium?

So I want to run an experiment with yeast. I want to monitor the population as close as possible in regard to its size. I am aware I could use a colorimeter or such, but I can only get a microscope. ...
Brian Blumberg's user avatar
1 vote
1 answer
151 views

What would "N" and "V" stand for in DNA barcoding?

I'm currently looking protocol for PAT-seq, and when it talks about the barcoding that's utilized, the documentation mentions there being an "N" and "NN" in the sequence, as well as a "V". For example:...
user avatar
1 vote
1 answer
3k views

How can I measure the population growth of yeast?

I will soon have to carry out an experiment. I want to estimate the rate of growth of a population of yeast, although I don't know if it is possible to at least estimate the number of yeast bacteria ...
Brian Blumberg's user avatar
0 votes
1 answer
386 views

How does the OD correlate with the number of algae cells?

My research team and I are using a spectrometer to measure the OD of an algae sample. We are trying to answer the question "Is there a correlation between OD and the number of algae cells?" What ...
AProNoob's user avatar
10 votes
3 answers
2k views

Why did scientists think humans had 100,000 genes (before the Human Genome Project)?

One of the major results of the Human Genome Project (HGP) was that humans have far fewer separate genes than previously thought. From a 2004 article about the HGP: Francis S. Collins, director of ...
tel's user avatar
  • 3,781
-1 votes
1 answer
85 views

Are there situations where in vivo results work better than in vitro results would have shown?

Basically, if I have poor in vitro cell line results but decided to test them in vivo in a mouse anyway what are the possibilities of having better results in the in vivo live mouse model that I would ...
Leon's user avatar
  • 101