I have two tissues and they are not treated; I would like just to compare the basal level of expression of the gene of interest (goi) in these two types of tissue (tissue1 and tissue2). After qPCR, I have the following result:
I wonder how am I supposed to make a decision. Will in this case goi be more expressed in
ctrl - is a housekeeping gene that we use for our types of tissue. Marked in yellow are just mean values. The last column is
mean goi_value - mean ctrl_value qPCR quality is not great.