So I have been working on a project for a while, and there is one mechanism that I need a little bit of advice about.
The project is chemical-biology based, so don't be deterred :)
Theoretically, by this point in the experiment:
I put cells (let's say lymphatic or endometrial) into stasis, through means of lyopreservation, and now, I have brought them out of stasis, through exposure to a sucrose (trehalose)-based medium.
Now I am attempting to chemically detect mitotic growth within the cells, how can I do this?
This is where the chemistry comes in: My first thought was to use a chromatic indicator that would fluoresce or irradiate in response to mitotic acceleration, but I have not yet found a suitable chemical. In research, I found that a similar experiment had been done in bacteria, and the chemical dimyristoylphosphatidylcholine (1,2-Dimyristoyl-sn-glycero-3-phosphocholine) had been used. Would this work in mammalian cells? Another idea was to use a chemical that detects the unnatural growth from the spindle fiber and centriole point of view, reacting when the centrioles entered the phase to replicate. I have been unable to find any chemicals that make use of the replication of centrioles, or the spindle fibers, so I need ideas.
To summarize: Does anybody know of any biochromatic indicators that react through detection of rapid, mammalian, mitotic growth? Not necessarily cancerous and uninhibited, but rapid meaning 'a speed of mitosis that is faster than the norm.' Preferrably, the indicator would be able to stay in stasis for extended periods of time.
If such an indicator is not available (or you do not believe so) then does there exist a biochromatic indicator that reacts in the presence of any sort of cell division?
Is there a way to chemically detect cell division?
I am welcome to all ideas.