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Tagged with flow-cytometry fluorescent-microscopy
3 questions
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How to correct the different (background) fluorescence for different cell types?
I am differentiating mouse bone marrow macrophages in vitro. However, I found that the differentiated macrophages have higher background fluorescence (the unstained sample) in all channels, which ...
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Can flow-FISH be used on metaphase cells?
I know flow-FISH can be used on interphase cells but was wondering if it only works on interphase cells or can work on cells regardless of stage in the cell cycle.
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Hoechst or DAPI for nuclear staining?
When is it best to use Hoechst vs. DAPI for nuclear staining? They seem to be very similar on paper. Are there situations where one is clearly preferable?
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