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Is the process of covalently link two DNA ends. The process is usually done by the enzyme class of ligases.
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Can someone link me to resources on the efficiency of sticky end ligation?
To measure the frequency of indels at the ligation site you can use a vector with a unique restriction site in the lacZ gene. With a colorimetric assay you can count the number of white cfu. … Perfect ligation in-frame yields blue cfu …