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Results tagged with pcr
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user 16900
Polymerase Chain Reaction is a method of replicating DNA exponentially from even a single molecule.
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Freezing after restriction digest
I usually purify the DNA (PCR inserts and linearised vector) using a kit or gel electrophoresis but today I don't have time for it. … Is it okay to freeze (-20*C) the PCR inserts and linearised vector following heat inactivation of the enzymes? After a week, I will thaw, purify and perform ligation on these products.
Thanks! …
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