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Results tagged with ligation
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user 4747
Is the process of covalently link two DNA ends. The process is usually done by the enzyme class of ligases.
3
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answer
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Can Bacteria Repair Dephosphorylated Plasmids?
The HinDIII version worked the first time, with few colonies on the empty vector control plate, and more colonies on the plates that received oligos during the ligation. … EDIT: I have the data for the transformations that skipped the ligation and SOC media incubation. Skipping the incubation did not change anything, neither did skipping the ligation. …
- 5,348
0
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Closing a Plasmid by Ligation
I have been having trouble with what should be a fairly simple ligation. I had a plasmid that I needed to cut part out of, so I designed a couple PCR primers to do that. … One idea I had was to do the PCR and then try blunt end ligation, since the Pfu polymerase should make blunt ends. Also got a new sample of ligase. …
- 5,348