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Andrew Roots
Andrew Roots
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Why How come SSBPs in RPA don't single-stranded binding proteins bind primers during recombinase polymerase amplification?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But why aren'thow come the primers aren't bound by SSBP, thus preventing them being bound by the recombinase? Why is itHow come it's not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequence.

What determines which single-stranded DNAsequences, how do these two enzymes "know" which single stranded DNA to bind to?

Why don't single-stranded binding proteins bind primers during recombinase polymerase amplification?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But why aren't primers bound by SSBP, thus preventing them being bound by the recombinase? Why is it not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequence.

What determines which single-stranded DNA these two enzymes bind to?

How come SSBPs in RPA don't bind primers?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But how come the primers aren't bound by SSBP thus preventing them being bound by the recombinase? How come it's not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequences, how do these two enzymes "know" which single stranded DNA to bind to?

Spelled out title. Corrected English and removed anthropomorphic implications
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David
David
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How come SSBPs in RPA Why don't single-stranded binding proteins bind primers during recombinase polymerase amplification?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But how come the primerswhy aren't primers bound by SSBP, thus preventing them being bound by the recombinase? How come it'sWhy is it not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequences, how do these two enzymes "know"sequence.

What determines which single stranded-stranded DNA tothese two enzymes bind to?

How come SSBPs in RPA don't bind primers?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But how come the primers aren't bound by SSBP thus preventing them being bound by the recombinase? How come it's not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequences, how do these two enzymes "know" which single stranded DNA to bind to?

Why don't single-stranded binding proteins bind primers during recombinase polymerase amplification?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But why aren't primers bound by SSBP, thus preventing them being bound by the recombinase? Why is it not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequence.

What determines which single-stranded DNA these two enzymes bind to?

Source Link
Andrew Roots
Andrew Roots
  • 36
  • 3

How come SSBPs in RPA don't bind primers?

I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, and the displaced strand of DNA is bound by single-strand binding protein (SSBP) to prevent reannealing and promote extension of the bound primer. But how come the primers aren't bound by SSBP thus preventing them being bound by the recombinase? How come it's not recombinase that binds the displaced DNA strand? Materials that I'm reading don't mention anything about adding some kind of recombinase-recognised tags to the primer sequences, how do these two enzymes "know" which single stranded DNA to bind to?