I've been growing bacteria on an agar plate and after putting the dishes in the incubator, this is what I saw. I'm new to bacteria identification, and is this just contamination by fungi or is this actually bacteria colonies? I suppose it is fungi, but what about the white, round white circles on top of the fungi(the top, left picture), is that bacteria colonies?
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1$\begingroup$ Well what did you put on the plate? Currently it's extremely hard to answer because we know very little about your experimental protocol. $\endgroup$– rg255Commented Apr 5, 2016 at 5:52
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$\begingroup$ @rg255 I put my thumb on the plate, to see the amount of bacteria on it to then compare the amount with after washing the hand using different methods. I suppose the plate may have been contaminated from the air because of insufficient sterile technique. $\endgroup$– Alexander ACommented Apr 6, 2016 at 7:19
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3$\begingroup$ How about a side view? Do you see spores in the stalks? $\endgroup$– WYSIWYGCommented Apr 11, 2016 at 6:46
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1 Answer
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It's probably mostly fungal contamination if you did not sterilize and left the container open. This video on how to isolate bacteria better may help: https://www.jove.com/video/3064/aseptic-laboratory-techniques-plating-methods
Good luck!