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I am collecting literature to start a new project on CRISPR/Cas9 gene editing. I must put together a protocol to start and am intending to use the following paper as guidance:

"Genome engineering using the CRISPR-Cas9 system". Nature Protocol 8(11):2281-308 · November 2013

Could you please indicate whether this is the most reliable and recent published general protocol for the CRISPR/Cas9 system.

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    $\begingroup$ A PubMed search will tell you that. $\endgroup$
    – MattDMo
    Commented May 3, 2016 at 20:08
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    $\begingroup$ Dear MattDMo, I am aware of that. There are many papers to choose from and go through all will take large amounts of time. What I am aiming for is to collect information from experienced people and optimize my time. Thanks $\endgroup$
    – Sergio.pv
    Commented May 4, 2016 at 5:11
  • $\begingroup$ Sergio - the way to do this is to search pubmed, and sort by date. Alternatively, email somebody from a lab who is currently performing CRISPR and ask them, usually people are happy enough to help with simple questions like that. $\endgroup$
    – Dermot Harnett
    Commented May 4, 2016 at 11:58
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    $\begingroup$ You can easily find out if it is most recent, if it is most reliable is an opinion-based question and as such out of the scope of this platform. $\endgroup$
    – skymningen
    Commented Dec 7, 2016 at 12:22

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It really depends on what you want to do with it. Sticking to genome engineering in Human cells, Nature Protocol 8(11):2281-308 · November 2013 is the de-facto standard. One improvement I suggest is to replace the "Surveyor Assay" with deconvolution analysis of sequencing traces using TIDE.

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