I am collecting literature to start a new project on CRISPR/Cas9 gene editing. I must put together a protocol to start and am intending to use the following paper as guidance:
"Genome engineering using the CRISPR-Cas9 system". Nature Protocol 8(11):2281-308 · November 2013
Could you please indicate whether this is the most reliable and recent published general protocol for the CRISPR/Cas9 system.