I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend several minutes. liver is soft but very small, and too hard to keep the weight. (human blood spend time for defreeze, but It degraded, too.)
for example, I want to weight 20mg mouse liver, 1. cut liver by scissors about 10mg after set zero point of scales with ep-tube. 2. cut liver almost 10mg, but it's exceed weight 2~3mg, so I have to cut it 2~3mg. 3. this scale is too small... so I spend for keep the weight for 20mg, like this: 10->23->19->19.3->19.8->21->19.8->20.
I want to ask for advice about how it faster animal tissue measuring and preparation. When preparation genomic DNA, is it OK so DNA is stable than RNA. but, when extract RNA, keep the weight fast and incubate sample at ice for prevent RNA degradation.