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RNA, or ribonucleic acid, is a biological macromolecule made of nucleotides used in cells to convey genetic information to protein. RNA also plays a role in catalyzing certain biological reactions as well as carrying genetic material in some viruses.

3 votes
1 answer
1k views

Why are riboswitches mostly present in bacteria and not in eukaryotes?

Riboswitches are a rather elegant way to regulate gene expression without any additional machinery. A small ligand binds to the mRNA and directly influences transcription or translation. Most of the …
Mad Scientist's user avatar
12 votes
1 answer
577 views

Which sequence characteristics influence the transcription efficiency of T7 polymerase?

The T7 polymerase doesn't transcribe all sequences equally well, the transciption efficiency can vary widely for different sequences. One well known requirement of the T7 polymerase is that the sequen …
Mad Scientist's user avatar
14 votes
1 answer
27k views

Why does RNA adopt an A-form helix?

RNA is known to form an A-form helix, while DNA generally forms a B-form helix under physiological conditions. From left to right: A-form DNA, B-form DNA, Z-form DNA. … Which interactions are important and cause RNA to adopt an A-form helix? …
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17 votes
4 answers
7k views

What implications has the missing 2'-OH on the capability of DNA to form 3D structures?

The major effect of that change that I know of is the higher stability of DNA compared to RNA. … "A DNA enzyme that cleaves RNA". Chem Biol. 1 (4): 223–9 …
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