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Results tagged with staining
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user 96
Set of methods to visualize certain properties (structure, gene expression, potential changes, etc.) of organisms or single cells.
11
votes
1
answer
751
views
Are these Gram stain substitutions acceptable?
In the context of a Gram stain on a blood smear:
Are the following acceptable substitutions and/or what differences could arise by substituting them?
Using methylene blue instead of crystal violet …
- 641
7
votes
Alternatives to ethidium bromide for staining small nucleic acids?
I would think GelRed or GelGreen would be an option too. They claim to be more sensitive than EtBr and certainly less toxic (even moreso than SYBR Green). I haven't personally used them against such …
- 585
3
votes
What conditions should I use for Gel Red staining?
I have only done this with Post-staining. 1-2.5% agarose with TAE. Used a 10,000x solution from Phenix research. The gelred stock and working solution needs to kept in the dark. … That being said there are a ton of things that can go wrong in casting the gel, eletrophoresis, staining, and visualization. …
- 585