I need the map of the pMON7124 plasmid. As this is not easy to find, I welcome any hints.
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$\begingroup$ perhaps contact these people (ncbi.nlm.nih.gov/pmc/articles/PMC237841/pdf/…) as these seems to be one of the original papers working with the plasmid. $\endgroup$– Behzad RowshanravanCommented Aug 13, 2014 at 20:05
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$\begingroup$ Addgene is a good site to find plasmids $\endgroup$– WYSIWYGCommented Aug 14, 2014 at 8:12
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It seems that no good map of this plasmid is around. Life technology uses it in some of its bacterial strains, the quote:
E. coli also contain the helper plasmid, pMON7124 (13.2 kb), which encodes the transposase and confers resistance to tetracycline. The helper plasmid provides the Tn7 transposition function in trans.
They link to the original publication, but the image quality in there is pretty bad:
They give some information in the text, so I would recommend reading the paper (if you have no access, let me know, I can help here):
- A broad-host-range shuttle system for gene insertion into the chromosomes of gram-negative bacteria.
If this is not enough, you could still try to get a better version from the author Gerard F. Berry (you can find his Monsanto email adress by googling for it).
