1
$\begingroup$

Specifically, I'm trying to find the sequence of BxB1 and its att sites. I have success finding the att site sequences, but I'm having a bit more of an issue with finding the sequence of the actual recombinase. I've looked through Genebank, but I can't find a definitive sequence of the BxB1 recombinase.

How should I go about looking for this sequence?

EDIT: One more thing I'm concerned about is the validity of the sequences that I do find. For example, I have seen that Addgene has this pCAG-NLS-HA-Bxb1 sequence. However, I'm not sure how to verify that this sequence is correct.

$\endgroup$
  • 1
    $\begingroup$ The genome has the gene annotated. This plasmid has the gene. $\endgroup$ – canadianer Jun 29 '15 at 23:17
1
$\begingroup$

http://phagesdb.org/phages/Bxb1/ here you can click "Locally BLAST this genome" it will give you the sequence in FASTA format and then you can click BLAST if you want additional information about the sequence, alignment, etc

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.