I am new to these concepts in biology and need some help understanding. My main concern is how to find the enhancer of my gene of interest, specifically the sequence of the enhancer. I am working on a project where I will be attempting to use CRISPR- mediated deletion of the promoter region and enhancer region in my gene of interest FOXN2, in MCF7 Tamoxifen resistant breast cancer cells. I already have the sequence for the promoter region from UCSC's GenomeBrowser, but I'm not sure if the enhancer region sequence can just be looked up on there or anywhere else. Do I need to do a protocol to find the enhancer region myself? I have seen many things about ChIP-Seq and that I may need to do that to get data to find my enhancer but I am just not sure. I plan to use the sequences of the enhancer and promoter region of my gene of interest to create gRNA for CRISPR. Thank you to anyone who can help.

  • $\begingroup$ Encode is a good resource for browsing cis-regulatory elements in the human and mouse genomes. Here is the page listing candidate cis-regulatory elements proximal to the human FOXN2 gene. $\endgroup$ – Dirigible Nov 19 '19 at 1:08
  • $\begingroup$ Remember that genes can have several enhancer and repressor regions and they can be spread more or less all over the genome if you're unlucky. FOXN2 seems to be reasonably well-known though, so there should be information on this out there. $\endgroup$ – Armatus Nov 19 '19 at 11:31

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