When human-rodent hybrids are made the amount of human chromosome in the hybrid can be reduced by irradiation. Why and how exactly is this irradiation step performed?
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$\begingroup$ "Why and how exactly is it performed?" Are you specifically asking about irradiation? $\endgroup$– JamesCommented Aug 25, 2015 at 17:37
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$\begingroup$ Im asking chiefly why it is done $\endgroup$– Naveen KumaarCommented Aug 25, 2015 at 17:40
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$\begingroup$ And the principle behind it $\endgroup$– Naveen KumaarCommented Aug 25, 2015 at 17:41
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$\begingroup$ I've made an edit that tries to specify that a bit better. $\endgroup$– JamesCommented Aug 25, 2015 at 17:49
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Human cells are irradiated with X rays (to fragment the DNA) and then fused with rodent cells. This is useful for mapping because closely linked markers are more likely to appear in the same hybrid: there is a lower probability of radiation induced breakage between the markers because they are close together.
answered Aug 25, 2015 at 19:14