Sorry for the possibly confused question, my knowledge of genetics is limited to medical training only but I have a question.

Are gene editing techniques such as CRISPR used on both of the chromosomes in a pair or just on one of them?

Some of the general searching I have done suggests that it can hit one or both. Is there a preferred outcome?


1 Answer 1


Gene editing using CRISPR-Cas9 can result in both heterozygous and homozygous conditions. It is just the event of probability. But even if u get heterozygous line, making it homozygous is not very difficult. You just have to cross two heterozygous line and screen for homozygous line through markers.

  • $\begingroup$ Thank you very much for the response. In general is it preferred to gain a homozygous or a hetrozygous change or is it specific to the intended task? $\endgroup$
    – Maxmansung
    Jan 3, 2019 at 22:23
  • $\begingroup$ It is specific to the task. If homozygous is not lethal, then i would go for homozygous line, as it will be easy to maintain. In case if you maintain heterozygous, then screening at each generation is necessary. $\endgroup$
    – RKK
    Jan 4, 2019 at 12:49

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