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Results tagged with rna
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user 5630
RNA, or ribonucleic acid, is a biological macromolecule made of nucleotides used in cells to convey genetic information to protein. RNA also plays a role in catalyzing certain biological reactions as well as carrying genetic material in some viruses.
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RNA integrity and RNA fragmentation
After RNA isolation and before following experiments, I know that RNA quality so called RNA integrity is so important. So why there is a RNA fragmentation kit? Do we need RNA intact or fragmented? … (RNA will be used for biotinylation and RT-qPCR afterwards) …
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Dilution question about RNA
I am isolating RNA with TRIzol. I have performed the basic steps and dissolved the pellet in 50 uL water. I ve measured the concentration and it was 3313 ng/uL (Around 165 ug total yield.). … It says 1 unit of enzyme for 1 ug of RNA in 10 ul reaction mix. I would like to dilute my RNA to 100 ug and use 100 units of enzyme in a 1000 uL reaction mix. …
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Does biotin affect downstream experiments, such as RT-qPCR?
I have two biotins--one of which is binding my RNA with cleavable bonds, and the other with irreversible bonds. Does biotin have an effect if it keeps its bonds with RNA? …
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RNase and EtOH issue
I work with RNA and I have some RNase issues. For example I am using RNeasy mini kit and at one step I use 100% EtOH. It does not say it is RNase free or not. …
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Do RNA molecules bind each other?
Let's assume that we isolated some RNA from a tissue. Do RNA molecules can bind each other if they have the complementary sequence? … I know that some small RNA molecules can bind other RNAs and regulate them but I do not know it is possible in this case? It is like 2 different mRNA can bind each other or not? …
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RNA isolation from Drosophila head
I need to isolate RNA from Drosophila head. … I use TRIzol RNA isolation for larvae brain a lot and have no problem with the method itself. However, with adult I got really low RNA concentration which is weird. …
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Elution of biotinylated RNA
Do you think it would effect my RNA? I have isolated my RNA with TRIzol which also contains Guanidine salt but the concentration is not that high. … As a second question, do you know other ways to elute my biotinylated RNA from beads?
Thanks! …
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A question related to qPCR analysis
I am using a method, called TU-Tagging, to isolate cell type specific RNA. … So, when 4-TU provided externally, UPRT incorporates 4-TU into RNA and you get thio-RNA. Then with basic biotinylation and streptaividin isolation you can get thio-RNA from your total RNA isolate. …
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In vitro transcription, contamination problem
I am using a RNA which is in vitro transcripted before I started my project. It turned out it is not prepared properly and has DNA contamination. … And second thing, in vitro transcipted RNA is in different dilutions, so I have a little bit of stock (maybe 5 uL) which is around 200 ng/uL and I have other dilutions 1 and 0.1 ng/uL. …
- 413
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Ribosomal RNA amount in a Drosophila cell
I am isolation RNA from Drosophila larvae brain with TRIzol method. What percentage of extracted RNA will be ribosomal RNA? …
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