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Results tagged with staining
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user 948
Set of methods to visualize certain properties (structure, gene expression, potential changes, etc.) of organisms or single cells.
4
votes
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Forgot to vortex antibody before staining
It depends on how much time elapsed between adding the concentrated antibodies to the diluting solution and adding the diluted antibodies to the slide. If it was just a couple of seconds, then I can a …
- 15.3k
3
votes
Accepted
Staining cells for FACS at 4 degrees or ambient temperature
The purpose of staining at 4°C and washing with cold buffer is twofold - to maintain viability (all cellular processes slow down at lower temps) and to prevent receptor internalization. … L/D should always be the last staining step before running your samples. …
- 15.3k