I don't think it's possible for TAQ to be stored with the primers, but I'm not sure. This storage wouldn't be long-term (a few days, or a week at most). Thoughts?

  • 2
    $\begingroup$ I have kept PCR stock solutions containing everything except DNA template for days to weeks at 4°C, and it works fine, at least for screening purposes (say, E. coli colony PCR or purified plasmid templates). $\endgroup$
    – gaspanic
    Dec 22 '20 at 19:10
  • $\begingroup$ @gaspanic -20C would be ideal, though, especially for the dNTPs. $\endgroup$
    – MattDMo
    Dec 22 '20 at 23:45
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    $\begingroup$ @MattDMo dNTPs are perfectly stable for months at 4°C, see for example here and here. I always keep an aliquot of my dNTP stock solution at 4°C both for convenience, but also to avoid repeated freeze-thaw cycles. $\endgroup$
    – gaspanic
    Dec 23 '20 at 12:27
  • $\begingroup$ @gaspanic that's good to know, thanks $\endgroup$
    – MattDMo
    Dec 23 '20 at 19:46

Yes, this is perfectly fine. Many companies sell PCR Master Mixes (granted, without primers):

If you choose to make your own, make sure it's stored at -20°C, and be sure to aliquot it so it's not going to be subjected to multiple freeze/thaw cycles. Obviously you should mix it well before aliquoting, but even after thawing I would vortex it to make sure all of the components are well-mixed. Make a 2X master mix, and adjust to a final 1X concentration in the PCR tube with your DNA sample and nuclease-free H2O.


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