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Questions relating to protocols, procedures, and good practice when using laboratory equipment.

5 votes

Are bleach solutions still routinely used in biochemistry laboratories to rid surfaces of ba...

I was associated with a lab in 2015 that used a lot of Streptococcus pneumoniae. They had ethanol squirt bottles to clean certain surfaces and in case of a spill. This was intended to kill that bacter …
Polypipe Wrangler's user avatar
1 vote

Reliable recipe to make DIY yeast slants

Yeast grows very well in liquid. The initial innoculum from the store will visible grow (“prove”) in warm water with a little sugar.
Polypipe Wrangler's user avatar
1 vote

How does the choice of blood draw site influence the possible specificity of a serological t...

This ref, https://academic.oup.com/ajcp/article/144/6/885/1761216 show that drops of finger prick blood vary more than venous blood. The main reason for using venous blood is licensing. A test is lic …
Polypipe Wrangler's user avatar
1 vote

How pricey does the media need to be for a typical bacterial culture experiment?

This answer only concerns Streptococcus pneumoniae. The lab I worked in used cheap ingredients, the same as everyone else claimed to in this field. The only shocking thing I found was that the steril …
Polypipe Wrangler's user avatar
1 vote

How should I measure the oxygen dispersed during photosynthesis in pondweeds?

Bubble formation might be easier to measure than oxygen concentration. Bubbles can be measured optically Or acoustically. A sealed container with a little carbon dioxide gas might work. See below arti …
Polypipe Wrangler's user avatar