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Questions tagged [diy-biology]

DIY biology is a movement of hobby biologists practicing outside of mainstream labs. This means that budget, availability of technology, and possibly background knowledge may be minimal.

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1answer
17 views

How to supply oxygen to a culture vessel?

I'm reading this paper: https://www.jstage.jst.go.jp/article/jpsa/51/3/51_0130043/_pdf From day 17 of the culture period until hatching, pure oxygen was supplied at a flow rate of approximately 500 ...
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2answers
96 views

DIYbio - CRISPR injection sites for targeting the ABCC11 gene [closed]

I've been researching into the biohacking world where people most notable Josiah Zayner and Tristan Roberts have used a CRISPR solution developed in their backyard for gene therapy. There is even a ...
2
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1answer
41 views

Why electroporator need a cuvette?

I have never obtain an Electroporator for my home-lab. Because it's very expensive, I'm trying to create a diy cheap version for myself. In all commercial design, I see that we need to put the sample ...
1
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1answer
148 views

Mold identification

I poured some agar plates and tried inoculating them with mushroom spores (I don't think they germinated bc of low humidity), but noticed what looks like mold with crystals on the surface of the agar. ...
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1answer
826 views

Beginner question about growing E. coli

I have bought a hobbyist kit which involves growing E. coli. The steps said to grow the E. coli on an LB agar Petri dish overnight. No incubation devices were included in the kit. I let the E. coli ...
6
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2answers
633 views

How to obtain a tardigrade?

The tardigrade is often mentioned as one of the most "alien" lifeforms on Earth. This video speaks for itself: (from this article) I have long wanted to see a tardigrade with my own eyes. I have a ...
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1answer
856 views

Can genetic engineering be performed without a laboratory setting? [closed]

I'm becoming more interested in genetic engineering as I learn more about it, and it's becoming more and more apparent that there's very few resources on genetic engineering at home. A similar ...
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1answer
217 views

Real Time PCR Test Chemistry

I am working on building a real time PCR machine. Is there any chemistry set that I can purchase online to: Identify if PCR amplification was successfully done. Test fluorescence dyes that could be ...
4
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2answers
158 views

Detecting food fraud

There's undoubtedly more than one way to do this, but if a DIY biologist were to attempt to detect food fraud (e.g. as done by students from Stanford University and Trinity School, Manhattan with ...
15
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1answer
15k views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
1
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1answer
220 views

Preparation of normal DNA polymerase

i don't need Taq polymerase. Just need normal DNA polymerase. So the main idea is preparation polymerase directly from Lactobacillus without using cloned vector. My idea is electrophoresis using ...
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2answers
246 views

Finding a easy and cheap method for dyeing dNTP

I want to measure OD to know the concentration of dNTP. Any idea for dyeing dNTP at cheapest price and easiest way ?
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2answers
2k views

Beginning with centrifuge experiment

This is the first time I do a centrifuge experiment with my own centrifuge machine, it's just simple made by a rotor that can rotate the tube at high speed. As I know a centrifuge machine can be using ...
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1answer
1k views

DIY extraction and long term storage of human DNA sample?

What is the recommended DIY way of extracting and long term storing of human DNA samples to store at home and send for sequencing in years time? Considerations: Sample material: cheek swab, hair, ...
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2answers
97 views

Some question about materials for PCR

I'm planning to order oligo, polymerase, nucleotide, buffer for my first diy PCR experiment (now I have nothing), so I have some questions. How and how long I can storage them? I have only a fridge ...
6
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2answers
603 views

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?
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3answers
1k views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
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3answers
183 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
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3answers
183 views

Pump to separate protein/DNA instead of electrophoresis?

I know that DNA molecules or proteins can be separated in electrophoresis because the electrical charge is used to pull the DNA through the gel. So instead of using electrical, can I using physical ...
4
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1answer
347 views

Effect of doubling volumes of PCR reagents

We ran PCR with a positive control and two lanes of sample. We used the same sample, but in one PCR mixture we used a total volume of 50μL (25μL Taq, 5μL forward&reverse primer each, 10&...