All Questions
Tagged with transformation yeast
6 questions
0
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1
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Gene of interest number of copies in transformed K. pastoris
When transforming a K. pastoris strain, is there a way to predict how many copies of the gene will be integrated following the homologous recombination? More specifically is it something that a ...
- 1
1
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0
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174
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Yeast transformation plate confluent with growth
I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
- 11
2
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0
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70
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What is the maximal insert length for PCR based homologous recombination in S. cerevisae
I would like to insert a 6 kbp construct, which I have on a plasmid into the genome of S. cerevisiae. This plasmid was originally constructed to integrate at the HIS locus via homologous recombination ...
- 21
3
votes
1
answer
2k
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What is the best current understanding of how yeast transformation works?
I would like to get myself up to speed with what is currently known to science about yeast transformation. Specifically, transformation of plasmids and linear DNA fragments. I am particularly ...
- 4,540
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1
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How the concentration of an antibiotic is chosen for YPD medium?
I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
- 95
11
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2
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Why is PEG important for efficient yeast transformation?
One way to do an yeast transformation is by using lithium acetate, a single-stranded carrier DNA, and PEG (1). I was wondering why is the polyethylene glycol important for the efficient transformation....
- 6,935