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Results tagged with molecular-biology
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user 540
The study of the molecular processes underlying life.
3
votes
Accepted
When should you use a stringent plasmid?
The three general advantages are:
Lower expression. If your plasmid is expressing something toxic to cells at high levels, reducing the amount of DNA can reduce expression.
Lower plasmid burden. If …
- 4,540
4
votes
1
answer
131
views
What's up with all the vague protocols? [closed]
I have lost count of how many protocols I've seen, including those supposed to be professionally written (such as manuals that come with kits from well known brands, or methods sections of papers in r …
- 4,540
10
votes
Accepted
What is the difference between second and third generation sequencing
I'm assuming you mean DNA sequencing (excluding things like RNA-seq).
Is Sanger sequencing the first generation?
From Metzker 2010:
The automated Sanger method is considered as a "first-generati …
- 4,540
8
votes
1
answer
19k
views
What is the definition of a stringent/relaxed plasmid?
I have found a publication which proposes some definitions, including a definition for strict and relaxed replication. The definitions are:
Relaxed control of plasmid replication. Relaxed control sho …
- 4,540
3
votes
1
answer
2k
views
What is "bacto" peptone?
Standard recipes for yeast medium often include "bacto-peptone". Is this the same as bacteriological peptone? Is there an authoritative source that spells it out?
- 4,540
2
votes
1
answer
490
views
What are the Gateway clonase enzymes?
The Gateway cloning system utilizes what Life Technologies refers to as "Clonase enzyme mix" to catalyze the BP and LR reactions.
What is in this enzyme mix? Is there a sequence for them?
- 4,540
2
votes
Accepted
Is AT or GC content important in electrophoresis?
It does make a difference on polyacrylamide. A and C are faster while G and T are slower.
Image from publication:
The "standards" are AAA/TTT/GGG/CCC molecules.
- 4,540