All Questions
Tagged with gene-synthesis synthetic-biology
7 questions
11
votes
3
answers
3k
views
DNA synthesis companies: cost per base, turn-around time, codon-optimization algorithms
I would like to synthesize a 3.4kb gene (originally isolated from soil bacteria) and transform it into E. coli. There are several companies (DNA2.0, GeneScript, BlueHeron) which will synthesize the ...
- 6,935
9
votes
1
answer
217
views
How realistic is to use DNA for long term storage?
This is mainly a followup question to the recent paper Next-Generation Digital Information Storage in DNA.
Personally, while I agree about the data density of the format, I can't help point out the ...
- 7,877
5
votes
1
answer
176
views
Do DNA supply houses maintain a watch list for dangerous oligos (Anthrax, Smallpox, etc.)?
The recent work by DeLoache, et al. on a synthetic opiate-precursor production pathway in yeast has generated a lot of scare stories in the media about people homebrewing heroin as easily as they ...
- 3,781
4
votes
1
answer
187
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Replacing, instead of repairing, DNA
I've been doing some light reading on DNA damage theory of aging. One of the main ideas from that theory that I got is that the accumulation of damage in our DNA is one of the biggest causes of why we ...
- 229
3
votes
2
answers
198
views
Dealing with Repetitive Bases in DNA?
I have been given a project involving a plasmid that contains long stretches of Adenine (60 or 120 bases each). These PolyA stretches are interrupted by the occasional G or C.
I understand that ...
- 5,348
2
votes
1
answer
115
views
Is synthesizing DNA in-house feasible?
I've been reading up about DNA writing, and it seems there are machines available around the five-figure US dollar mark for DNA synthesis, and I'm considering picking one up for my lab.
We normally ...
-1
votes
1
answer
67
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How would it be possible to engineer cows to have more offspring?
Cows usually only give birth to one calf per year and only several in their lifetime. What would we have to genetically alter so they can have more offspring, more frequently. I read that their is a ...
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