Questions tagged [orf]

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5
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1answer
74 views

How to determine the most likely reading frame of a DNA sequence?

This question is from a past exam paper for an introductory bioinformatics module. I'm a computer scientist doing biology for the first time. "A short bacterial gene has been sequenced, giving the ...
3
votes
2answers
5k views

Why are there six reading frames if only one strand of DNA is referred to as the ‘coding strand’?

We need to consider six reading frames when considering the potential of DNA to encode protein (three frames for each strand). But only one strand is transcribed into RNA — the so-called coding strand....
3
votes
1answer
221 views

How to interpret Glimmer scores?

I am trying to find orfs with Glimmer. At the end, Glimmer gives me a .predict file with the orfs and a corresponding raw score. When I look at the documentation, it says about the score, "This is ...
1
vote
2answers
208 views

Which ORF will be translated

I've a question about ORF and translation into protein. Say I have an RNA transcript that contains two ORFs, one in phase 1 ; one in phase2 ; as: ORF number 1 in reading frame 1 on the direct strand ...
5
votes
1answer
350 views

How do biologists infer correct ORF of a DNA sequence?

Each DNA (RNA) sequence has 6 possible Open Reading Frames(ORF). My question is: What are the theoretical bases of in vitro or in silico tries to find correct reading frame of a sequence? Is it just ...