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Questions tagged [orf]

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3
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Why are there six reading frames if only one strand of DNA is referred to as the ‘coding strand’?

We need to consider six reading frames when considering the potential of DNA to encode protein (three frames for each strand). But only one strand is transcribed into RNA — the so-called coding strand....
3
votes
1answer
182 views

How to interpret Glimmer scores?

I am trying to find orfs with Glimmer. At the end, Glimmer gives me a .predict file with the orfs and a corresponding raw score. When I look at the documentation, it says about the score, "This is ...
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vote
2answers
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Which ORF will be translated

I've a question about ORF and translation into protein. Say I have an RNA transcript that contains two ORFs, one in phase 1 ; one in phase2 ; as: ORF number 1 in reading frame 1 on the direct ...
5
votes
1answer
343 views

How do biologists infer correct ORF of a DNA sequence?

Each DNA (RNA) sequence has 6 possible Open Reading Frames(ORF). My question is: What are the theoretical bases of in vitro or in silico tries to find correct reading frame of a sequence? Is it just ...