All Questions
Tagged with transformation dna
6 questions
3
votes
1
answer
651
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Plasmid Design and Integration events [Single vs Double cross over]
When linearising a vector by restriction digest within the middle of a homologous region can a single cross over integration event only occur if the plasmid is re-ligated within the cell after ...
- 638
6
votes
2
answers
103
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Which method of gene amplification for toehold switches?
My team and I are from a high school and are planning to carry out some research investigating some toehold switch riboregulators which we have designed in silico. However, we have little experience ...
- 634
4
votes
1
answer
281
views
How does the DNA cross through bacterial cell wall during electroporation?
There exists a lot of literature on electroporation of Gram-positive and negative bacteria. Most of it gives an explanation that electroporation works by creating transient pores in cell membranes of ...
- 5,683
1
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1
answer
688
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Heat shock vs electroporation
I've been transforming E. coli via heat shock in order to insert oligonucleotides (around 50 nt); however, none of my experiments have given positive results so far. I begin to question the efficiency ...
CommunityBot
- 1
2
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0
answers
398
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Transfect Mammalian Cells with Single Stranded DNA?
Agrobacteria can deliver parts of their DNA to plant cells as T-DNA, transfer DNA. This DNA is delivered as a single strand and can be integrated into the plant genome or can be converted to a double ...
2
votes
1
answer
749
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How does electroporation mediated recombination work?
For yeast and other organisms, DNA can be readily taken up into the cell naturally or through membrane disruption. What does applying a voltage to cells do exactly that allows more recombination to ...
CommunityBot
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