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1 vote

modify PCR steps to include a ligation

In case this helps anyone, I found a technique called Gap Ligase Chain Reaction which includes both a thermostable ligase and a DNA polymerase enzyme in the same reaction: Ligase Chain Reaction (LCR) -...
Ryan's user avatar
  • 121
1 vote

Separate DNA fragments with very different size (oligo and lambda-DNA)

A spin column might work for your purposes since you can do multiple rounds of binding if your volume is too large to bind in a single pass, but for DNA of that size a lot will be irreversibly stuck ...
tyersome's user avatar
  • 5,598
1 vote

Ligation without purifying insert

Thanks all for your comments guys. I tried purifying using PCR purification kit. I could recover 120 ng of purified fragment from 500 ng. It is ok for my further work.
RKK's user avatar
  • 630

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