3
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I accidentally plated DH5a E. coli and left them in the 4℃ for 12 hours, if I put them into the 37℃ incubator will they start growing?
If these were just regular E. coli that you were growing up, then it wouldn't matter too much - they would certainly grow a lot slower on the plate than you might otherwise expect compared to a plate ...
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1
vote
Accepted
modify PCR steps to include a ligation
In case this helps anyone, I found a technique called Gap Ligase Chain Reaction which includes both a thermostable ligase and a DNA polymerase enzyme in the same reaction:
Ligase Chain Reaction (LCR) -...
- 121
1
vote
Separate DNA fragments with very different size (oligo and lambda-DNA)
A spin column might work for your purposes since you can do multiple rounds of binding if your volume is too large to bind in a single pass, but for DNA of that size a lot will be irreversibly stuck ...
- 5,683
1
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Ligation without purifying insert
Thanks all for your comments guys. I tried purifying using PCR purification kit. I could recover 120 ng of purified fragment from 500 ng. It is ok for my further work.
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