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RNA, or ribonucleic acid, is a biological macromolecule made of nucleotides used in cells to convey genetic information to protein. RNA also plays a role in catalyzing certain biological reactions as well as carrying genetic material in some viruses.
9
votes
Accepted
Where do amino acids get attached to tRNA and where is it synthesized?
A pre-tRNA is transcribed from tRNA genes in DNA by RNA polymerase III. … You'll find there's a unique splicing mechanism in tRNA that additionally splices out an anticodon intron which is abesnt in mature tRNA's:
The wikipedia article notes RNA Pol III generally recognizes …
4
votes
Accepted
How do cells determine RNA types?
Rather, the gene has the elements, just like it does to select the RNA Pol it needs, to direct the sort of modification the RNA needs to obtain. … The image below depicts two types of RNA transcribed by RNA Pol II and how the processing elements let the modification machinery know how to handle them:
Source …
1
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How to isolate host cell RNA (tRNA,mRNA,rRNA) from viral RNA?
Thermo Fisher has a protocol for the separation of host mammalian RNA from prokaryotic RNA, optimized for E. coli vs human, mouse or rat sequences. … Otherwise, Thermo Fisher has additional links to RNA isolation protocol.. most of the viral assays, however, utilize serum, plasma, etc. because host DNA/RNA will elute along with the viral RNA if they're …
1
vote
H3K36me3 role in RNA polymerase II elongation
Looking at yeast studies, though, it would seem that RNA Pol II is capable of transcribing through heterochromatin anyways so long as specific cofactors are present (Acetylation of H3 K56 Is Required for … RNA Polymerase II Transcript Elongation through Heterochromatin in Yeast) …
1
vote
Accepted
What is meant by "Expression" of Non-Coding RNA?
They queried publications dealing with the lncRNA that studied whether it was functional through over-expression or knockdown. Cells do something measurable in their normal state, that can be observed …
0
votes
Why is 5S-rRNA is different from other rRNAs in place of transcription and usage of RNA poly...
This article suggests RNA Pol III formed early in eukaryote evolution, and while there's a lot of conservation, the enzyme-specific subunits result from divergent evolution. …
0
votes
What physical force attracts the anti-codon on tRNA to the codon on mRNA during translation?
I wanted to illustrate the process I explained in a comment on aandreev's answer with some images, I just copy/pasted the actual text sections, however.
From comments:
The tRNA is bound to an elonga …