Questions tagged [transformation]
The genetic alteration of a cell resulting from the direct uptake, incorporation, and expression of exogenous genetic material from its surroundings.
10 questions with no upvoted or accepted answers
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Generate T2/T1 Phage Resistant E Coli
I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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How much Kanamycin is too much for selection in dh5α E. coli?
I'm using Kanamycin for selection after electroporation of my plasmid containing a kanamycin-resistance gene at a concentration of 50mg/L prepared in 1000x stock that is usually kept in frozen ...
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CRISPR Knock in
Using the CRISPR/Cas9 technology, it is possible that after inducing a DSB with the Cas9 endonuclease guided with an RNA designed by the user and using a template DNA, get a desired Knock-In (KI) by ...
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What is the maximal insert length for PCR based homologous recombination in S. cerevisae
I would like to insert a 6 kbp construct, which I have on a plasmid into the genome of S. cerevisiae. This plasmid was originally constructed to integrate at the HIS locus via homologous recombination ...
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Transfect Mammalian Cells with Single Stranded DNA?
Agrobacteria can deliver parts of their DNA to plant cells as T-DNA, transfer DNA. This DNA is delivered as a single strand and can be integrated into the plant genome or can be converted to a double ...
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Yeast transformation plate confluent with growth
I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
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Has anyone tried to prepare chemically competent Mycobacteria?
Bacterial species like E. coli are easy to transform using a variety of methods, including both chemical transformation and electroporation. Chemical transformation is especially nice to use as it can ...
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Griffith's Experiment Conclusions
My teacher gave us these two questions on Griffith's experiment.
How did Griffith's experiment rule out that the rough cells could have used the capsules of the smooth cells to become pathogenic?
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Questions regarding transformation in bacterial cells
First off, in transformation the donor DNA aligns itself with the complementary bases in the recipient DNA.
Now a "perfect" alignment of the donor DNA ( Sorry if my terminologies are ...
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Does prokaryotic transcription have activators / enhancer regions involved?
I am designing a biosensor, and I need to know whether prokaryotic transcription involves or can involve (if a gene needs to be regulated) enhancer regions. Also, where are enhancer regions located (...
