Questions tagged [elisa]
ELISA stands for enyzme-linked immunosorbent assay. It is analytical method which binds an antigen to an antibody in a microreaction plate und uses a second, enzyme-linked antibody for the detection.
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Troubleshooting direct sandwich ELISA
I am validating an ELISA measuring A1c (also called glycated hemoglobin; target for humans) for use in another species. It is a direct sandwich assay. The standard and my sample (whole blood) were ...
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Need help designing an enzyme immunoassay (EIA)
I have a FITC-labelled DNA sequence and an anti-FITC antibody, however this antibody is not conjugated with HRP or any other enzyme. I work in a lab with limited finances, so is there a way I could ...
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Sensitivity vs. Limit of Detection of rapid antigen tests
I'm comparing a bunch of SARS-CoV2 rapid antigen tests:
Source
Columns 4 and 6 list the values for sensitivity and limit of detection (LOD). How come that a test with a several times lower limit of ...
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What could cause individual false positives in ELISAs, while the rest of the plate looks fine?
I run indirect ELISAs for COVID antibodies. That's my whole job right now as a research technician. I coat the plates with a pure RBD, then detect antibodies in serum. Recently it's come to light that ...
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Converting from RU/ML to AEU/ML
I'd like to know whether it is possible to convert from RU/ml to AEU/ml.
I work in the ELISA department in our laboratory. We need to validate some kits by comparing results between our lab and ...
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TMB vs ECL for ELISA: which detection method is more sensitive?
I failed to find a comparison, does anyone know which ELISA detection method is more sensitive of these two: ECL, TMB?
Many thanks!
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Why can't we always create a vaccine against a virus when an ELISA test to detect it is possible?
Before precising my question, here are some facts that I presume to be true:
A vaccine works by injecting the antigens of a virus into the body to train the immune system to recognize the virus and ...
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Why there is no ELISA for SARS-CoV-2?
As far as I know, SARS-CoV-2 tests currently used worldwide are real-time RT-PCR. Why there is no ELISA for SARS-CoV-2?
Compared to PCRs, ELISA is:
Way cheaper;
Way faster;
Does not require trained ...
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ELISA troubleshooting
Hi,
Why does my elisa plate wells gives a brown color after adding the stop solution. I am giving a 4*5 minutes wash after each step. TMB gives it a proper blue color but when I add the stop solution ...
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What is the composition of a standard diluent buffer in a leptin ELISA kit?
I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...
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Getting Started with ELISAs
I'm interested in designing an ELISA to detect the presence of Aflatoxin B1, but I'm having a hard time finding information about designing an enzyme and some sort of guidance on how ELISA protocols ...
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Can experiments in ELISA kits be monitored via both fluorometry and photometry?
In my bioanalytics course slides, the professor has written at one point that in a heterogenous immunoassay such as ELISA, we use fluorimetry to measure concentration of an analyte. In another slide ...
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Calculating CV for intra-assay ELISA
Sorry if this is in the wrong thread.
I have to calculate CV for intra-assay ELISA sample OD's. My problem is that i'm finding people are using sample standard deviation in their CV formula, though ...
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the range of interleukin 2 and 12 that can be used to activate nk-92 cells
I am one of the biotech students. I want to know the dynamic ranges of interleukin 2 and 12 that i can use to activate the NK-92 Cells.
thank you
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Concentration calculation of Factor VIII for Elisa
I have the following problem. I'm trying to get the concentration calculation for an Excel sheet that will calculate values from an Elisa plate.
The basis is a standard curve with the following ...
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Rationale behind washing off unbound protein antigen in indirect ELISA
My question is with regards to the usability of the indirect ELISA in measuring protein concentration. Suppose we want to quantify the concentration of a protein. The indirect ELISA method would ...
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About sandwich ELISA
In sandwich ELISA the Fc region of primary antibody bind to the polystyrene coated well. But what are the specific interactions (like 'hydrophobic interaction' or 'van der waals force') happen between ...
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Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme
If the goal is to generate a rapid assay for an enzyme of plant source what are the typical options?
i.e. Could one do something like: Generate an antibody to the enzyme and then use it to create an ...
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What is the advantage of indirect ELISA over direct one?
I guess the answer is about indirect one giving less error due to selectivity but how exactly does that happen?
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Background correction when reading ELISA with TMB substrate
I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
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Identifying whether ELISA kits are authentic or fake
What methods can be used to determine whether they are authentic or fake?
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How to use mechanical microstrainer to extract tissue proteins from human? [closed]
Background:
There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
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What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?
I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources:
Thermo Scientific ...
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