New answers tagged experimental-design
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A more useful question to ask would be, Why do you place the disc on the plate before the bacteria have grown up? This test allows us to infer which antibiotics an organism is susceptible and resistant to by observing whether or not they grow in the presence of that given antibiotic.
But there's a good reason we don't wait until the bacteria are already ...
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Personal anecdote: Once I planned to sterilize seeds of Silene latifolia for tissue culture but after the first step (washing the seeds in water for an hour) I had to do other things and the seeds did spend the night wet in room temperature. The next day some of the seeds were showing first signs of germination - a little crack in seed coat. I decided to ...
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