Questions tagged [plasmids]
Often circular segments of DNA that can replicate outside of the chromosomes. Generally found in bacteria.
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questions with no upvoted or accepted answers
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Are there any alternatives to the Epicentre product Plasmid Safe?
I need to remove any traces of linear DNA (both single and double stranded) from a ligation reaction while keeping circular DNA intact. Up to now, I have used Epicentre's Plasmid Safe to do the job. I ...
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Gibson assembly - primer design with A and T rich regions
I have question about Gibson assembly. I have done it several times and it always worked okay for us, but now I want to assemble together a fragment which has sequence like this: 5'...
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When doing minipreps, do “good” plasmids produce more DNA than “bad” plasmids?
I've been doing minipreps for close to 10 years using the standard qiagen kits and DH5a e. coli. I typically do 24 minipreps at a time and then identify the good plasmids by enzyme digestion, PCR, ...
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Can you Interrupt a promoter region by inserting another promoter region into it?
I currently have a plasmid (pFPV25.1) which contains a promoter region with multiple cloning sights in it. I would like to place a new promoter I have isolated, and I was wondering whether I could ...
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Why is there a size limit on inserts that a plasmid can accept?
Throughout my undergraduate education I have been taught that plasmids can't carry very large inserts, but I have never been told why. Any insight would be greatly appreciated.
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When purifying plasmid DNA after cloning, do you get endogenous plasmid in addition to your vector of interest?
Since bacteria naturally contain plasmids, when you do a cloning experiment and purify the plasmids at the end, do you get plasmid naturally present in that bacteria in addition to your vector? The ...
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Which plasmid can express T7 RNA polymerase in mammalian cells?
Does anyone know what plasmid could express T7 RNA polymerase in mammalian cells, I would like to transfect it with other plasmid containing Gene Of Interest under control of T7 promoter. Thanks a lot!...
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What is the origin of plasmids?
As I understand it, plasmids, like mitochondria, have their own genetic material and are capable of self-replication.
According to Wikipedia: Plasmids are considered replicons, units of DNA capable ...
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Plasmid harbouring subunit S of type I R/M system
We have an assembly of a genome of a Lactobacillus species. The genome also contains a 7.5kb plasmid.
Aside from the "usual plasmid genes" the plasmid contains:
- toxin/antitoxin system
- Subunit S ...
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Is it possible to both extract DNA sequences from plasmids and fuse them using primers for Gibson assembly?
Maybe I'm overcomplicating this, but I'm having some issues with a construct.
So, I have three plasmids containing a total of eight DNA sequences that I need to fuse into one linear sequence. I plan ...
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102 views
How to specify a promoter in de novo gene synthesis service?
I am trying to use a de novo gene synthesis service (Genscript) and one part confuses me:
Where do I pick the choice of promoter? Or is the promoter choice automatic based on my plasmid choice?
e.g....
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Commercial suppliers of cloned vectors?
I am a recent transplant from academia to industry. I would like to follow-up on some experiments in the literature, for which I need cloned plasmids. Addgene of course doesn't (for the most part) ...
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Pet 28a plasmid is obtained from which type of bacterial strain
I want to ask some question which is very confusing
Pet28a is a vector.I want to know which strain of bacteria have pet28a plasmid?
E.coli DH5 alpha strain have pet28a plasmid?
Please help me .......
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24 views
Induce plasmid acquisition in bacteria to make them susceptible to antibiotics
Is it possible to use plasmids as a way of creating antibiotic susceptibility in bacteria.
For example; in Microbiology, an introduction 13th edition by Pearson , Page 230 "the researchers traced the ...
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74 views
Join a linear plasmid just using a primer (without ligase enzyme)
Can I use just a primer and PCR to join the cutted plasmid like this ? without using any further enzyme like ligase.
After the linear dsDNA plasmid annealed into 2 ssDNA, primer will bind to middle. ...
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History of plasmid maps
To create a small history of visual representations in biology, I am seeking the earliest published papers or books that have used plasmid maps as figures ; or strategies to effectively search for ...
